Anti-Presenilin 1/PS-1 (phospho S357) antibody (ab78914)
Key features and details
- Rabbit polyclonal to Presenilin 1/PS-1 (phospho S357)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Presenilin 1/PS-1 (phospho S357) antibody
See all Presenilin 1/PS-1 primary antibodies -
Description
Rabbit polyclonal to Presenilin 1/PS-1 (phospho S357) -
Host species
Rabbit -
Specificity
ab78914 detects endogenous levels of Presenilin 1/PS-1 only when phosphorylated at serine 357 (Human: Ser357; Mouse: Ser357; Rat: Ser358).
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human Presenilin 1/PS-1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab78914 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 (phospho S357) antibody (ab78914)
ab78914 at 1/50 dilution staining Presenilin 1/PS-1 in human brain by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
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Western blot - Anti-Presenilin 1/PS-1 (phospho S357) antibody (ab78914)All lanes : Anti-Presenilin 1/PS-1 (phospho S357) antibody (ab78914) at 1/500 dilution
Lane 1 : RAW264.7 cell extracts,
treated with UV (5mins)
Lane 2 : RAW264.7 cell extracts,
treated with UV (5mins) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
