Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1515Y] to Presenilin 2/AD5
  • Suitable for: ICC, WB, IHC-P, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Presenilin 2/AD5 antibody [EP1515Y]
    See all Presenilin 2/AD5 primary antibodies

  • Description

    Rabbit monoclonal [EP1515Y] to Presenilin 2/AD5

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Rat
    IHC-P
    Human
    IP
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Presenilin 2/AD5 aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, HepG2, C2C12, C6, and Neuro-2a lysates; IHC-P: Human cerebrum tissue; ICC: PC-12 cells; Flow Cyt: HeLa cells; IP: HeLa and PC-12 cell lysates.

  • General notes

    Previously labelled as Presenilin 2. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
    Western blot - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
    All lanes : Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249) at 1/20000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 3 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
    Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 5 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 50 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Presenilin 2/AD5 with purified ab51249 at 1/500 dilution (0.51 µg/ml). Heat mediated antigen retrieval using BondTM Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
    Immunocytochemistry - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
    Immunocytochemistry/ Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling Presenilin 2/AD5 with purified ab51249 at 1/50 dilution (5 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
    Flow Cytometry - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Presenilin 2/AD5 with purified ab51249 at 1/50 dilution (5 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
    Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

    ab51249 (purified ) at 1/20 dilution (1ug) immunoprecipitating Presenilin 2/AD5 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab51249 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
    Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

    Presenilin was immunoprecipitated from 1 mg of HeLa whole cell extract with unpurified ab51249 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab51249 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Input: HeLa whole cell extract 10 µg.

     IP (+): ab51249 IP in HeLa whole cell extract.

     IP (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in HeLa whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

  • Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)
    Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)

    Presenilin was immunoprecipitated from 1 mg of PC-12 (rat adrenal gland pheochromocytoma) whole cell extract with unpurified ab51249 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab51249 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Input: PC-12 whole cell extract 10 µg.

    IP (+): ab51249 IP in PC-12 whole cell extract.

    IP (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in PC-12 whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

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