Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 16, 2021

Anti-AKR1C3 antibody [EPR16725] (ab203834)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16725] to AKR1C3
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-AKR1C3 antibody [EPR16725]
See all AKR1C3 primary antibodies
Description

Rabbit monoclonal [EPR16725] to AKR1C3
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal brain, fetal kidney and fetal liver lysates; MOLT-4 cell lysate. IP: Human fetal liver whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16725
Isotype

IgG
Research areas

Western blot - Anti-AKR1C3 antibody [EPR16725] (ab203834)

Anti-AKR1C3 antibody [EPR16725] (ab203834) at 1/5000 dilution + Human fetal brain lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 37 kDa
Observed band size: 37 kDa

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-AKR1C3 antibody [EPR16725] (ab203834)

Anti-AKR1C3 antibody [EPR16725] (ab203834) at 1/10000 dilution + MOLT-4 (Human lymphoblastic leukemia cell line) cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 37 kDa
Observed band size: 37 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-AKR1C3 antibody [EPR16725] (ab203834)

All lanes : Anti-AKR1C3 antibody [EPR16725] (ab203834) at 1/10000 dilution

Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal liver lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 37 kDa
Observed band size: 37 kDa

Exposure time: 3 minutes

Blocking buffer: 5% NFDM/TBST.
Dilution buffer: 5% NFDM /TBST or 1%BSA /TBST.
Immunoprecipitation - Anti-AKR1C3 antibody [EPR16725] (ab203834)

AKR1C3 was immunoprecipitated from 1mg of Human fetal liver whole cell lysate with ab203834 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203834 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: Human fetal liver whole cell lysate 10ug (Input). Lane 2: ab203834 IP in Human fetal liver whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203834 in Human fetal liver whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
Anti-AKR1C3 antibody [EPR16725] (ab203834)

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