Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20277] to Folate Binding Protein/FBP
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-Folate Binding Protein/FBP antibody [EPR20277]
See all Folate Binding Protein/FBP primary antibodies
Description

Rabbit monoclonal [EPR20277] to Folate Binding Protein/FBP
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal kidney, mouse kidney, and rat kidney tissue lysates and JAR, and HeLa cell lysates. IHC-P: Human kidney, ovarian cancer and lung cancer tissues; mouse kidney tissue; rat kidney tissue.
General notes
Previously labelled as Folate Binding Protein
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20277
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051, ready to use). Cytoplasmic staining on human kidney (PMID: 22204844). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051, ready to use).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)

All lanes : Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FOLR1 knockout HeLa cell lysate
Lane 3 : JAR cell lysate
Lane 4 : MDA-MB-231 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 30 kDa
Observed band size: 38 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab221543 observed at 38 kDa. Red - loading control, ab7291 observed at 52 kDa.

ab221543 Anti-Folate Binding Protein/FBP antibody [EPR20277] was shown to specifically react with Folate Binding Protein/FBP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264921 (knockout cell lysate ab257270) was used. Wild-type and Folate Binding Protein/FBP knockout samples were subjected to SDS-PAGE. ab221543 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)

All lanes : Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543) at 1/5000 dilution

Lane 1 : GST-His-tagged human recombinant FOLR1 (aa 25-160), 20 ng
Lane 2 : His-tagged human recombinant FOLR2 (aa 24-154), 20 ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 30 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?

Exposure time:

Lanes 1-2: 5 seconds
Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)

All lanes : Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543) at 1/1000 dilution

Lane 1 : JAR (human placenta choriocarcinoma epithelial cells) whole cell lysate at 20 µg
Lane 2 : Human fetal kidney lysate at 20 µg
Lane 3 : Mouse kidney lysate at 20 µg
Lane 4 : Rat kidney lysate
Lane 5 : HeLa (Human cervix adenocarcinoma epithelial cells) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 30 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?

Exposure times:

Lane 1:10 seconds

Lane 2: 30 seconds

Lanes 3-4: 5 seconds

Lane 5: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)

Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use). Mainly membranous staining on tumor cells of human ovarian cancer (PMID: 22204844). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use). Cytoplasmic staining on tumor cells of human lung cancer (PMID: 22204844). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use). Positive staining on mouse kidney (PMID: 22204844). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use). Positive staining on rat kidney (PMID: 22204844). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)

Negative control:

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use). No staining on human tonsil (PMID: 22547449). Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)

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