Palmitoylated Protein Assay Kit (Green) (ab273282)
Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Fluorescent
- Platform: Flow cytometer, Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
-
Product name
Palmitoylated Protein Assay Kit (Green)
See all Palmitoylated Protein kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Product overview
Palmitoylated Protein Activity Assay Kit (Green) (ab273282) is a highly specific, simple and robust method for labeling and detection of palmitoylated proteins. The kit uses a modified Palmitic Acid that is fed directly into the cells and gets incorporated into proteins during or post translation. This post translational modification can be followed by click reaction with an azide-containing dye. The assay kit offers a powerful method for imaging localization, trafficking, and dynamics of Palmitoylated proteins or detection by FACS for quantitative studies.
-
Tested applications
Suitable for: Flow Cyt, FMmore details -
Platform
Flow cytometer, Fluorescence microscope
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Copper Reagent (100X) 1 x 100µl Fixative Solution 1 x 10ml Fluorescent Azide (100X) 1 x 100µl Palmitic Acid Label (1000X) 1 x 10µl Permeabilization Buffer (10X) 1 x 25ml Reducing Agent (20X) 1 x 500µl Total DNA Stain (1000X) 1 x 20µl Wash Buffer (10X) 1 x 25ml
Images
-
Metabolic labeling of palmitic acid on proliferating Jurkat cells
Metabolic labeling of palmitic acid on proliferating Jurkat cells. 1 x 106 cells incubated overnight with fresh aliquots of media containing Palmitic Acid Label. Analysis of Negative Control (Black), Background (Fluorescent Azide only, Green), Positive Control (Palmitic Acid Label and Fluorescent Azide, Pink). Signal measured in FL-1 channel clearly shows the Palmitic Acid Labeling of protein.
-
Metabolic labeling of palmitic acid on proliferating HeLa cellsMetabolic labeling of palmitic acid on proliferating HeLa cells. 1 x 105 cells were incubated overnight with fresh aliquots of media containing Palmitic Acid Label. Upper panel corresponds to the Azide only Background fluorescence. The lower panel shows green fluorescence corresponds to the Palmitic Acid Label labeling. Nuclear staining in both panels confirms that green signal is a result of Palmitic Acid Label incorporation.