Biotin Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab271253)
Key features and details
- Biotin Rabbit polyclonal to Macrophage Inflammatory Protein 1 alpha / CCL3
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Overview
-
Product name
Biotin Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody
See all Macrophage Inflammatory Protein 1 alpha / CCL3 primary antibodies -
Description
Biotin Rabbit polyclonal to Macrophage Inflammatory Protein 1 alpha / CCL3 -
Host species
Rabbit -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA Recombinant fragmentWB Recombinant fragment
-
Immunogen
Recombinant fragment corresponding to Human Macrophage Inflammatory Protein 1 alpha/ CCL3 aa 1 to the C-terminus.
Database link: P10147
Properties
-
Form
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Biotin Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab271253)
Western blot analysis of varying levels of Recombinant human MIP-1α protein using ab271253 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant MIP-1α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24 -
Sandwich ELISA - Biotin Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab271253)Human MIP-1α was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271253. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant MIP-1α protein.