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Immunology Innate Immunity Macrophage / Inflamm.

Rat GPNMB Antibody Pair - BSA and Azide free (ab253315)

Rat GPNMB Antibody Pair - BSA and Azide free (ab253315)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Rat

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Overview

  • Product name

    Rat GPNMB Antibody Pair - BSA and Azide free
    See all GPNMB kits
  • Assay type

    ELISA set
  • Range

    0.313 ng/ml - 20 ng/ml
  • Species reactivity

    Reacts with: Rat
  • Product overview

    The Antibody Pair can be used to quantify Rat GPNMB. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab270219), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Rat GPNMB Capture Antibody (unconjugated) 1 x 100µg
    Rat GPNMB Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
  • Function

    Could be a melanogenic enzyme.
  • Tissue specificity

    Not restricted to the melanocytic lineage.
  • Sequence similarities

    Belongs to the PMEL/NMB family.
    Contains 1 PKD domain.
  • Developmental stage

    Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines.
  • Cellular localization

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession Q14956 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Glycoprotein (transmembrane) nmb
    • Glycoprotein nmb
    • Glycoprotein nmb like protein
    • Gpnmb
    • GPNMB_HUMAN
    • HGFIN
    • NMB
    • Osteoactivin
    • Transmembrane glycoprotein
    • Transmembrane glycoprotein HGFIN
    • Transmembrane glycoprotein NMB
    see all
  • Database links

    • Entrez Gene: 113955 Rat
    • SwissProt: Q6P7C7 Rat
    • Unigene: 13778 Rat

    Images

    • Sandwich ELISA - Rat GPNMB Antibody Pair - BSA and Azide free (ab253315)
      Sandwich ELISA - Rat GPNMB Antibody Pair - BSA and Azide free (ab253315)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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