Rat GPNMB ELISA Kit (ab270219)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 136 pg/ml
- Range: 0.31 ng/ml - 20 ng/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat GPNMB ELISA Kit
See all GPNMB kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 7 4.3% -
Sample type
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
136 pg/ml -
Range
0.31 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 104 94% - 122% Serum 98 95% - 101% Cell culture media 112 109% - 114% Hep Plasma 105 94% - 121% EDTA Plasma 102 97% - 112% Cit plasma 120 112% - 133% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat GPNMB ELISA Kit (ab270219) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GPNMB protein in cell culture media, cell culture supernatant, edta plasma, serum, cit plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat GPNMB with 136 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
GPNMB (Glycoprotein non-metastatic gene B), also known as Osteoactivin, is a type I transmembrane glycoprotein most often associated with endosomal/lysosomal compartment and over expressed in many types of cancer. The GPNMB is involved in many physiological processes, including transport of late melanosomes to keratinocytes, regulation of osteoblast and osteoclast, stimulation of dendritic cell maturation, and stimulation of adhesion of dendritic cells to endothelial cells.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat GPNMB Capture Antibody 1 x 600µl 10X Rat GPNMB Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Plate Seals 1 unit Rat GPNMB Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Could be a melanogenic enzyme. -
Tissue specificity
Not restricted to the melanocytic lineage. -
Sequence similarities
Belongs to the PMEL/NMB family.
Contains 1 PKD domain. -
Developmental stage
Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines. -
Cellular localization
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Alternative names
- Glycoprotein (transmembrane) nmb
- Glycoprotein nmb
- Glycoprotein nmb like protein
see all -
Database links
- Entrez Gene: 113955 Rat
- SwissProt: Q6P7C7 Rat
- Unigene: 13778 Rat
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat GPNMB standard curve in Sample Diluent NS.The GPNMB standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native GPNMB in rat serum, plasma and cell culture supernatant samples.The concentrations of GPNMB were measured in duplicates, interpolated from the GPNMB standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 20%, plasma (citrate) 20%, plasma (EDTA) 40%, plasma (heparin) 20% and spleen supernatant 80%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GPNMB concentration was determined to be 59 ng/mL in neat serum, 48 ng/mL in neat plasma (citrate), 41 ng/mL in neat plasma (EDTA), 69 ng/mL in neat plasma (heparin) and 24 ng/mL in neat spleen supernatant.
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Sandwich ELISA - Rat GPNMB ELISA Kit (ab270219)To learn more about the advantages of recombinant antibodies see here.
