Mouse MCP2 Antibody Pair - BSA and Azide free (ab241739)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
Overview
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Product name
Mouse MCP2 Antibody Pair - BSA and Azide free
See all MCP2 kits -
Assay type
ELISA set -
Range
3.9 pg/ml - 250 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
The Antibody Pair can be used to quantify Mouse MCP2 (CCL8). BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab203366), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse MCP2 Capture Antibody (unconjugated) 1 x 100µg Mouse MCP2 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. -
Tissue specificity
Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Ccl8
- CCL8_HUMAN
- HC14
see all -
Database links
- Entrez Gene: 20307 Mouse
- SwissProt: Q9Z121 Mouse
- Unigene: 42029 Mouse
Images
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Sandwich ELISA - Mouse MCP2 Antibody Pair - BSA and Azide free (ab241739)To learn more about the advantages of recombinant antibodies see here.
