Mouse MCP2 Matched Antibody Pair Kit (ab213464)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 3.9 pg/ml - 250 pg/ml
Overview
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Product name
Mouse MCP2 Matched Antibody Pair Kit
See all MCP2 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
1.05 pg/ml -
Range
3.9 pg/ml - 250 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse MCP2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse MCP2.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab203366. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IA, Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse MCP2 Capture Antibody 2 x 50µg 1 x 50µg Mouse MCP2 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse MCP2 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. -
Tissue specificity
Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Ccl8
- CCL8_HUMAN
- HC14
see all -
Database links
- Entrez Gene: 20307 Mouse
- SwissProt: Q9Z121 Mouse
- Unigene: 42029 Mouse
Images
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Mouse MCP2 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse MCP2 Matched Antibody Pair Kit (ab213464)To learn more about the advantages of recombinant antibodies see here.
