Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free (ab251455)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20036] to CXCL7/PBP - BSA and Azide free
  • Suitable for: WB, IP, IHC-Fr, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free
    See all CXCL7/PBP primary antibodies

  • Description

    Rabbit monoclonal [EPR20036] to CXCL7/PBP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251455 is the carrier-free version of ab206406. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251455 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free (ab251455)
    Western blot - Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free (ab251455)
    Anti-CXCL7/PBP antibody [EPR20036] (ab206406) at 1/5000 dilution + Mouse CXCL7/PBP active protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa
    Observed band size: 7 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    This data was developed using ab206406, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Frozen sections) - Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free (ab251455)
    Immunohistochemistry (Frozen sections) - Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free (ab251455)
    This data was developed using ab206406, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling CXCL7/PBP with ab206406 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on megakaryocytes and platelets of mouse spleen was observed. The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free (ab251455)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free (ab251455)
    This data was developed using ab206406, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CXCL7/PBP with ab206406 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on megakaryocytes and platelets of mouse spleen is observed [PMID:16391012]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free (ab251455)
    Western blot - Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free (ab251455)
    Lanes 1-2 : Anti-CXCL7/PBP antibody [EPR20036] (ab206406) at 1/2000 dilution
    Lanes 3-4 : Anti-CXCL7/PBP antibody [EPR20036] (ab206406) at 1/10000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Mouse plasma
    Lane 3 : Mouse platelet tissue lysate
    Lane 4 : Mouse serum

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 14 kDa
    Observed band size: 7 kDa why is the actual band size different from the predicted?



    This data was developed using ab206406, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 4 seconds; Lanes 3-4: 1 second.

    The molecular weight is consistent with what has been described in the literature: PMID: 14673015.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free (ab251455)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free (ab251455)
    This data was developed using ab206406, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CXCL7/PBP with ab206406 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on platelets of mouse lung is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free (ab251455)
    Immunoprecipitation - Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free (ab251455)
    This data was developed using ab206406, the same antibody clone in a different buffer formulation.CXCL7/PBP was immunoprecipitated from 0.35 mg of Mouse spleen lysate with ab206406 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206406 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Mouse spleen lysate, 10 μg (Input). Lane 2: ab206406 IP in Mouse spleen lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206406 in Mouse spleen lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second.
  • Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free (ab251455)
    Anti-CXCL7/PBP antibody [EPR20036] - BSA and Azide free (ab251455)

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