Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)

Key features and details

  • Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Isotype: IgG1

Overview

  • Product name

    Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4]
    See all COX1 / Cyclooxygenase 1 primary antibodies

  • Description

    Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Other Immunogen Type corresponding to COX1/ Cyclooxygenase 1. A preparation of intracellular proteins of the human cell line HL60.

  • Positive control

    • WB: Huh-7 whole cell lysate. COX1 protein standard prepared from ram seminal vesicles. Flow Cytometry: NIH/3T3 cells. IHC-P: Human anal cancer tissue. Mouse kidney tissue. IHC-Fr: Human breast and breast tumor tissue. IP: Huh-7 whole cell lysate.

  • General notes

    Please note that we have received reports from customers working with this antibody that they obtain two stong bands in Western blot. A single band at around 72kDa is expected. One customer reported that when tested with three different human cell lines two strong bands are obtained, one between 40 - 50kDa and other other between 62 - 83kDa. It is possible that a degraded form of the enzyme is detected or the bands may correspond to different glycosylation states.

Properties

Images

  • Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)
    Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)

    Overlay histogram showing NIH/3T3 (Mouse embryo fibroblast cell line) cells stained with ab695 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab695, 2 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

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