Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319)
![Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319)](https://www.abcam.com/ps/products/133/ab133319/Images/ab133319-146825-anti-cox1-cyclooxygenase-1-antibody-epr5867-immunohistochemistry.jpg "Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5867] to COX1 / Cyclooxygenase 1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867]
See all COX1 / Cyclooxygenase 1 primary antibodies -
Description
Rabbit monoclonal [EPR5867] to COX1 / Cyclooxygenase 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic peptide within Human COX1/ Cyclooxygenase 1 aa 500-600. The exact sequence is proprietary.
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Positive control
- C2C12, A431, NIH 3T3, Neuro 2a, and L6 cell lysates; Human stomach tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.00 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5867 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319)
Immunohistochemical analysis of Paraffin Embedded Human stomach tissue labelling COX1/Cyclooxygenase 1 with ab133319 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319)](https://www.abcam.com/ps/products/133/ab133319/Images/ab133319-146826-anti-cox1-cyclooxygenase-1-antibody-epr5867-western-blot.jpg)
Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319)All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319) at 1/1000 dilution
Lane 1 : C2C12 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : NIH 3T3 cell lysate
Lane 4 : Neuro 2a cell lysate
Lane 5 : L6 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 68 kDa
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![OI-RD Scanning - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319)](https://www.abcam.com/ps/products/133/ab133319/Images/ab133319-146824-anti-cox1-cyclooxygenase-1-antibody-epr5867.jpg)
OI-RD Scanning - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319) -
![Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319)](https://www.abcam.com/ps/products/133/ab133319/Images/ab133319-4-benefits-of-recombinant-antibodies.png)
Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319)
