HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203) at 1/5000 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 69 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?


Exposure time: 4 minutes

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199203 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203) at 1/5000 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 69 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?


Exposure time: 2 minutes

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199203 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203) at 1/5000 dilution + C2C12 (Mouse skeletal muscle myoblast cell line) Whole Cell Lysate at 10 µg

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 69 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?


Exposure time: 30 seconds

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199203 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.