Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10708(B)] to WDR77 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free
    See all WDR77 primary antibodies

  • Description

    Rabbit monoclonal [EPR10708(B)] to WDR77 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human breast cancer and human testis tissue; ICC: 293T cells; Flow Cyt (intra): HeLa cells. WB: HeLa, HEK-293 whole cell lysate. Mouse kidney and spleen lysate and Rat lysate.

  • General notes

    ab249087 is the carrier-free version of ab154190.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Images

  • Western blot - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)
    Western blot - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)
    All lanes : Anti-WDR77 antibody [EPR10708(B)] (ab154190) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : LnCAP cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 37 kDa



    This data was developed using ab154190, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)
    Immunocytochemistry - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)

    This data was developed using ab154190, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/Immunofluorescence analysis of HT-29 (Human colorectal adenocarcinoma cell line) labeling WDR77 with Purified ab154190 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
  • Flow Cytometry - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)
    Flow Cytometry - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)

    This data was developed using ab154190, the same antibody clone in a different buffer formulation.

    Overlay histogram showing HeLa cells stained with ab154190 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab154190, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1 µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)

    This data was developed using ab154190, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling WDR77 with ab154190 at 1/50 dilution Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)
    This data was developed using ab154190, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling WDR77 with ab154190 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)
    This data was developed using ab154190, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue using ab154190 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)
    This data was developed using ab154190, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal testis tissue using ab154190 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)
    This data was developed using ab154190, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab154190 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)
    Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free (ab249087)

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