Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DCC antibody [EPR23313-115] - BSA and Azide free (ab273576)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23313-115] to DCC - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DCC antibody [EPR23313-115] - BSA and Azide free
    See all DCC primary antibodies

  • Description

    Rabbit monoclonal [EPR23313-115] to DCC - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    IHC-Fr
    Rat
    IHC-P
    Mouse
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human hippocampus tissue lysate; Mouse P5 hippocampus tissue lysate; Rat P7 hippocampus and Rat E18 brain tissue lysates. IHC-P: Human cerebrum and Mouse E14.5, Rat E14.5 tissues. IHC-Fr: Mouse E14.5 embryo and Rat E14.5 embryo tissue. Flow Cyt: Rat primary neuron cells. IP: Human hippocampus tissue lysate; Mouse P5 cerebral cortex tissue lysate.

  • General notes

    ab273576 is the carrier-free version of ab273570. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab273576 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCC antibody [EPR23313-115] (ab273576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCC antibody [EPR23313-115] (ab273576)

    Immunohistochemical analysis of paraffin-embedded Mouse E14.5 tissue labeling DCC with ab273570 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse E14.5 cerebrum (PMID: 24739528). The section was incubated with ab273570 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273570).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCC antibody [EPR23313-115] (ab273576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCC antibody [EPR23313-115] (ab273576)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling DCC with ab273570 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cerebrum (PMID: 11684721). The section was incubated with ab273570 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273570).

  • Immunohistochemistry (Frozen sections) - Anti-DCC antibody [EPR23313-115] (ab273576)
    Immunohistochemistry (Frozen sections) - Anti-DCC antibody [EPR23313-115] (ab273576)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat E14.5 embryo tissue labeling DCC with ab273570 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat embryonic brain is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273570).

  • Immunoprecipitation - Anti-DCC antibody [EPR23313-115] (ab273576)
    Immunoprecipitation - Anti-DCC antibody [EPR23313-115] (ab273576)

    DCC was immunoprecipitated from 0.35 mg Mouse P5 cerebral cortex tissue lysate  with ab273570 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273570 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse P5 cerebral cortex tissue lysate 10 ug

    Lane 2: ab273570 IP in Mouse P5 cerebral cortex tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273570 in Mouse P5 cerebral cortex tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273570).

  • Flow Cytometry - Anti-DCC antibody [EPR23313-115] (ab273576)
    Flow Cytometry - Anti-DCC antibody [EPR23313-115] (ab273576)

    Flow cytometric analysis of PC-12 (Rat adrenal gland pheochromocytoma, Left) / Rat primary neuron cells (Right) cells labelling DCC with ab273570 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: PC12 (PMID: 8044801).

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273570).

  • Immunohistochemistry (Frozen sections) - Anti-DCC antibody [EPR23313-115] (ab273576)
    Immunohistochemistry (Frozen sections) - Anti-DCC antibody [EPR23313-115] (ab273576)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse E14.5 embryo tissue labeling DCC with ab273570 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse embryonic brain is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273570).

  • Immunoprecipitation - Anti-DCC antibody [EPR23313-115] (ab273576)
    Immunoprecipitation - Anti-DCC antibody [EPR23313-115] (ab273576)

    DCC was immunoprecipitated from 0.35 mg Human hippocampus tissue lysate with ab273570 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273570 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human hippocampus tissue lysate 10 ug

    Lane 2: ab273570 IP in Human hippocampus tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273570 in human hippocampus tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273570).

  • Flow Cytometry - Anti-DCC antibody [EPR23313-115] (ab273576)
    Flow Cytometry - Anti-DCC antibody [EPR23313-115] (ab273576)

    Flow cytometric analysis of Rat primary neuron cells cells labelling DCC with ab273570 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273570).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCC antibody [EPR23313-115] (ab273576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCC antibody [EPR23313-115] (ab273576)

    Immunohistochemical analysis of paraffin-embedded Rat E14.5 tissue labeling DCC with ab273570 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat E14.5 cerebrum (PMID: 24739528). The section was incubated with ab273570 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273570).

  • Anti-DCC antibody [EPR23313-115] - BSA and Azide free (ab273576)
    Anti-DCC antibody [EPR23313-115] - BSA and Azide free (ab273576)

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