Antibodies, Proteins, Kits and Reagents for Life Science

Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19048] to MAT1A + MAT2A - BSA and Azide free
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free
See all MAT1A + MAT2A primary antibodies
Description

Rabbit monoclonal [EPR19048] to MAT1A + MAT2A - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251153 is the carrier-free version of ab192846. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251153 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR19048
Isotype

IgG

Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)

All lanes : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/20000 dilution

Lane 1 : Human MAT1A recombinant protein
Lane 2 : Human MAT2A recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 44 kDa
Observed band size: 44,75 kDa
why is the actual band size different from the predicted?

This data was developed using ab192846, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 1 second; Lane 2: 2 seconds.

Human MAT1A recombinant protein contains aa1-395 with a His-Tag^Â®. This recombinant protein was made in-house.
Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)

Lane 1 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution
Lanes 2-3 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/5000 dilution

Lane 1 : Human fetal liver tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Rat liver tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure time: 1 second

This data was developed using ab192846, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)

Lanes 1-3 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/10000 dilution
Lanes 4-6 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution

Lane 1 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : U937 (human histiocytic lymphoma cell line) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : Human fetal heart tissue lysate
Lane 5 : Human fetal kidney tissue lysate
Lane 6 : Human fetal spleen tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 4-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Developed using the ECL technique.

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure time: 3 seconds

This data was developed using ab192846, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)

All lanes : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution

Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat kidney tissue lysate
Lane 5 : C6 (rat glial tumor cell line) whole cell lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 44 kDa
Observed band size: 44 kDa

This data was developed using ab192846, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-2: 5 seconds; Lanes 3-6: 3 seconds.
Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)

All lanes : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/20000 dilution

Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) treated with 500 ng/ml Lipopolysaccharides for 4 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure time: 5 seconds

This data was developed using ab192846, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID: 18695670 and 22879628).
Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)

This data was developed using ab192846, the same antibody clone in a different buffer formulation.

MAT2A was immunoprecipitated from 0.35 mg of Ramos (human Burkitt's lymphoma cell line) whole cell lysate with ab192846 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab192846 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Ramos whole cell lysate 10 µg (Input).

Lane 2: ab192846 IP in Ramos whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab192846 in Ramos whole cell lysate.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 1 second.
Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)

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