Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19048] to MAT1A + MAT2A - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free
    See all MAT1A + MAT2A primary antibodies

  • Description

    Rabbit monoclonal [EPR19048] to MAT1A + MAT2A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251153 is the carrier-free version of ab192846. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251153 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR19048

  • Isotype

    IgG

Images

  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)
    Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)
    All lanes : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/20000 dilution

    Lane 1 : Human MAT1A recombinant protein
    Lane 2 : Human MAT2A recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44,75 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab192846, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 1 second; Lane 2: 2 seconds.

    Human MAT1A recombinant protein contains aa1-395 with a His-Tag®. This recombinant protein was made in-house.

  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)
    Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)
    Lane 1 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution
    Lanes 2-3 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/5000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Rat liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
    Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 1 second


    This data was developed using ab192846, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)
    Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)
    Lanes 1-3 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/10000 dilution
    Lanes 4-6 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution

    Lane 1 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : U937 (human histiocytic lymphoma cell line) whole cell lysate
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 4 : Human fetal heart tissue lysate
    Lane 5 : Human fetal kidney tissue lysate
    Lane 6 : Human fetal spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 4-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 3 seconds


    This data was developed using ab192846, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)
    Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)
    All lanes : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat kidney tissue lysate
    Lane 5 : C6 (rat glial tumor cell line) whole cell lysate
    Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa



    This data was developed using ab192846, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 5 seconds; Lanes 3-6: 3 seconds.

  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)
    Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)
    All lanes : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/20000 dilution

    Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) treated with 500 ng/ml Lipopolysaccharides for 4 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 5 seconds


    This data was developed using ab192846, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 18695670 and 22879628).

  • Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)
    Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)

    This data was developed using ab192846, the same antibody clone in a different buffer formulation.

    MAT2A was immunoprecipitated from 0.35 mg of Ramos (human Burkitt's lymphoma cell line) whole cell lysate with ab192846 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab192846 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Ramos whole cell lysate 10 µg (Input).

    Lane 2: ab192846 IP in Ramos whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab192846 in Ramos whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)
    Anti-MAT1A + MAT2A antibody [EPR19048] - BSA and Azide free (ab251153)

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