Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)
![Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)](https://www.abcam.com/ps/products/248/ab248206/Images/ab248206-3-anti-stat5a-phospho-s726-antibody-epr1914-2-bsa-and-azide-free-dot-blot.jpg "Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1914(2)] to STAT5a (phospho S726) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free
See all STAT5a primary antibodies -
Description
Rabbit monoclonal [EPR1914(2)] to STAT5a (phospho S726) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody only detects STAT5a phosphorylated at Serine 726.
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Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: FuncS,ICC,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab248206 is the carrier-free version of ab128896. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248206 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1914(2) -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)This data was developed using ab128896, the same antibody clone in a different buffer formulation.Primary antibody dilution: 1/1000 Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated Secondary antibody dilution: 1/2500 Blocking & dilution buffer: 5% NFDM/TBST Lane 1 sample: STAT5a (pS726) phospho peptide Lane 2 sample: STAT5a non-phospho peptide Exposure time: 10 seconds
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![Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)](https://www.abcam.com/ps/products/248/ab248206/Images/ab248206-403294-anti-stat5a-phospho-s726-antibody-epr1914-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)All lanes : Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896) at 1/5000 dilution
Lane 1 : SH-SY5Y (human neuroblastoma) treated with Okadaic acid and Calyculin A whole cell lysates
Lane 2 : Untreated SH-SY5Y (human neuroblastoma) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa
Exposure time: 3 minutesThis data was developed using ab128896, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST
> -
![Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)](https://www.abcam.com/ps/products/248/ab248206/Images/ab248206-399020-anti-stat5a-phospho-s726-antibody-epr1914-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)All lanes : Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysates: untreated
Lane 2 : SH-SY5Y cell lysates: treated with Okadaic acid and Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDaThis data was developed using ab128896, the same antibody clone in a different buffer formulation.
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![Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)](https://www.abcam.com/ps/products/248/ab248206/Images/ab248206-4-benefits-of-recombinant-antibodies.png)
Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)
