Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1914(2)] to STAT5a (phospho S726) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free
    See all STAT5a primary antibodies

  • Description

    Rabbit monoclonal [EPR1914(2)] to STAT5a (phospho S726) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects STAT5a phosphorylated at Serine 726.

  • Tested applications

    Suitable for: Dot blot, WBmore details
    Unsuitable for: FuncS,ICC,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248206 is the carrier-free version of ab128896. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248206 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)
    Dot Blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)
    This data was developed using ab128896, the same antibody clone in a different buffer formulation.Primary antibody dilution: 1/1000 Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated Secondary antibody dilution: 1/2500 Blocking & dilution buffer: 5% NFDM/TBST Lane 1 sample: STAT5a (pS726) phospho peptide Lane 2 sample: STAT5a non-phospho peptide Exposure time: 10 seconds
  • Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)
    Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)
    All lanes : Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896) at 1/5000 dilution

    Lane 1 : SH-SY5Y (human neuroblastoma) treated with Okadaic acid and Calyculin A whole cell lysates
    Lane 2 : Untreated SH-SY5Y (human neuroblastoma) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 90 kDa


    Exposure time: 3 minutes


    This data was developed using ab128896, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST

    >
  • Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)
    Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)
    All lanes : Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysates: untreated
    Lane 2 : SH-SY5Y cell lysates: treated with Okadaic acid and Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 90 kDa
    Observed band size: 90 kDa



    This data was developed using ab128896, the same antibody clone in a different buffer formulation.

     

  • Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)
    Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)

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