Anti-CD1d antibody [EPR22526-72] - BSA and Azide free (ab254464)
![Anti-CD1d antibody [EPR22526-72] - BSA and Azide free (ab254464)](https://www.abcam.com/ps/products/254/ab254464/Images/ab254464-1-229903IP1.jpg "Anti-CD1d antibody [EPR22526-72] - BSA and Azide free (ab254464)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22526-72] to CD1d - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-CD1d antibody [EPR22526-72] - BSA and Azide free
See all CD1d primary antibodies -
Description
Rabbit monoclonal [EPR22526-72] to CD1d - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: Jurkat whole cell lysate.
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General notes
Ab254464 is the carrier-free version of ab229903. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab254464 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22526-72 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-CD1d antibody [EPR22526-72] - BSA and Azide free (ab254464)
CD1d was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole lysate with ab229903 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229903 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: Jurkat whole cell lysate 10 µg (Input).
Lane 2: ab229903 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229903 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229903).
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![Anti-CD1d antibody [EPR22526-72] - BSA and Azide free (ab254464)](https://www.abcam.com/ps/products/254/ab254464/Images/ab254464-2-benefits-of-recombinant-antibodies.png)
Anti-CD1d antibody [EPR22526-72] - BSA and Azide free (ab254464)
