Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CD1d antibody [EPR22526-9] (ab256344)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22526-9] to CD1d
Suitable for: IP, WB, Flow Cyt
Reacts with: Human

Product name

Anti-CD1d antibody [EPR22526-9]
See all CD1d primary antibodies
Description

Rabbit monoclonal [EPR22526-9] to CD1d
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Jurkat and MOLT-4 whole cell lysate. IP: Jurkat whole cell lysate. Flow Cyt: Human peripheral blood mononuclear and K562 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22526-9
Isotype

IgG
Research areas

Western blot - Anti-CD1d antibody [EPR22526-9] (ab256344)

All lanes : Anti-CD1d antibody [EPR22526-9] (ab256344) at 1/1000 dilution

Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 2 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate
Lane 3 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 38 kDa
Observed band size: 48 kDa
why is the actual band size different from the predicted?

Exposure time: 37 seconds

The molecular weight observed is consistent with what has been described in the literature (PMID: 10809957).

Negative control: SH-SY5Y (PMID: 26749374), K562 (PMID: 21037579).

Blocking/Dilution buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-CD1d antibody [EPR22526-9] (ab256344)

Flow cytometric analysis of K562 (human chronic myelogenous leukemia lymphoblast, Left) / Jurkat (human T cell leukemia T lymphocyte, Right) cells labeling CD1d with ab256344 at 1/600 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

Negative control: K562 (PMID: 21037579).
Flow Cytometry - Anti-CD1d antibody [EPR22526-9] (ab256344)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CD1d with ab256344 at 1/600 dilution (0.1μg) (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150097) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab256344 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor^® 647. Gated on viable cells.
Immunoprecipitation - Anti-CD1d antibody [EPR22526-9] (ab256344)

CD1d was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with ab256344 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256344 at 1/1000 dilution (0.6 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

Lane 1: Jurkat whole cell lysate (10μg)
Lane 2: ab256344 IP in Jurkat whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256344 in Jurkat whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 mins.
Anti-CD1d antibody [EPR22526-9] (ab256344)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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