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Immunology Adaptive Immunity T Cells CD

Anti-TRAP/CD40L antibody [EP462E] (ab52750)

Price and availability

298 185 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-TRAP/CD40L antibody [EP462E] (ab52750)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP462E] to TRAP/CD40L
  • Suitable for: WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-TRAP/CD40L antibody [EP462E]
    See all TRAP/CD40L primary antibodies
  • Description

    Rabbit monoclonal [EP462E] to TRAP/CD40L
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with TRAP/CD40L. Mouse species is recommended by WB application. We have preliminary internal testing data to indicate this antibody may not react with mouse in IHC.
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human TRAP/CD40L aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: MOLT-4 cell lysate.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EP462E
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Immunoglobulins
    • Class Switching
    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Platelets
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Innate and adaptive immunity
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Images

  • Western blot - Anti-TRAP/CD40L antibody [EP462E] (ab52750)
    Western blot - Anti-TRAP/CD40L antibody [EP462E] (ab52750)
    All lanes : Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1 µg/ml

    Lane 1 : Human lymph node tissue lysate
    Lane 2 : Mouse lymph node tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Blocking and diluting buffer: 5% NFDM/TBST. 

    Exposure time: Lane 1: 90 seconds
                             Lane 2: 20 seconds
            

  • Western blot - Anti-TRAP/CD40L antibody [EP462E] (ab52750)
    Western blot - Anti-TRAP/CD40L antibody [EP462E] (ab52750)
    Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1/5000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa

  • Anti-TRAP/CD40L antibody [EP462E] (ab52750)
    Anti-TRAP/CD40L antibody [EP462E] (ab52750)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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