Anti-TRAP/CD40L antibody [EP462E] (ab52750)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP462E] to TRAP/CD40L
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-TRAP/CD40L antibody [EP462E]
See all TRAP/CD40L primary antibodies -
Description
Rabbit monoclonal [EP462E] to TRAP/CD40L -
Host species
Rabbit -
Specificity
This antibody reacts with TRAP/CD40L. Mouse species is recommended by WB application. We have preliminary internal testing data to indicate this antibody may not react with mouse in IHC. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Synthetic peptide within Human TRAP/CD40L aa 100-200. The exact sequence is proprietary.
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Positive control
- WB: MOLT-4 cell lysate.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP462E -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1 µg/ml
Lane 1 : Human lymph node tissue lysate
Lane 2 : Mouse lymph node tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDaBlocking and diluting buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 90 seconds
Lane 2: 20 seconds
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Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1/5000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
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