Anti-TRAP/CD40L antibody [EP462E] - BSA and Azide free (ab210743)
![Anti-TRAP/CD40L antibody [EP462E] - BSA and Azide free (ab210743)](https://www.abcam.com/ps/products/210/ab210743/Images/ab210743-293176-anti-trap-cd40l-antibody-ep462e-western-blot.jpg "Anti-TRAP/CD40L antibody [EP462E] - BSA and Azide free (ab210743)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP462E] to TRAP/CD40L - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-TRAP/CD40L antibody [EP462E] - BSA and Azide free
See all TRAP/CD40L primary antibodies -
Description
Rabbit monoclonal [EP462E] to TRAP/CD40L - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MOLT-4 cell lysate.
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General notes
ab210743 is the carrier-free version of ab52750 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab210743 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP462E -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRAP/CD40L antibody [EP462E] - BSA and Azide free (ab210743)Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1/5000 dilution + MOLT-4 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?This WB data was generated using the same anti-TRAP/CD40L antibody clone, EP462E, in a different buffer formulation (cat# ab52750).
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![Anti-TRAP/CD40L antibody [EP462E] - BSA and Azide free (ab210743)](https://www.abcam.com/ps/products/210/ab210743/Images/ab210743-2-benefits-of-recombinant-antibodies.png)
Anti-TRAP/CD40L antibody [EP462E] - BSA and Azide free (ab210743)
