Anti-Nucleoporin p62/NUP62 antibody (ab264365)
Key features and details
- Rabbit polyclonal to Nucleoporin p62/NUP62
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nucleoporin p62/NUP62 antibody
See all Nucleoporin p62/NUP62 primary antibodies -
Description
Rabbit polyclonal to Nucleoporin p62/NUP62 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human Nucleoporin p62/NUP62 aa 472-522. The exact sequence is proprietary.
Database link: P37198 -
Positive control
- IP: HEK-293T whole cell lysate. WB: HeLa, HEK-293T and Jurkat whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab264365)All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab264365) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 secondsCells prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Nucleoporin p62/NUP62 antibody (ab264365)Nucleoporin p62/NUP62 was immunoprecipitated from 1mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab264365 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264365 at 0.4 µg/ml.
Lane 1: ab264365 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.Exposure time: 30 secs.
Cells prepared using NETN lysis buffer.
