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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-Nucleoporin p62/NUP62 antibody [2A] (ab188413)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Nucleoporin p62/NUP62 antibody [2A] (ab188413)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rat monoclonal [2A] to Nucleoporin p62/NUP62
  • Suitable for: WB, ICC/IF
  • Reacts with: Human, African green monkey
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Nucleoporin p62/NUP62 antibody [2A]
    See all Nucleoporin p62/NUP62 primary antibodies
  • Description

    Rat monoclonal [2A] to Nucleoporin p62/NUP62
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, African green monkey
    Does not react with: Mouse
  • Immunogen

    Recombinant fragment (His-tag) corresponding to Human Nucleoporin p62/NUP62 aa 1-300. (N terminal proprietary tag).
    Database link: P37198

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Epitope

    aa 1-179 (FG-repeat region)
  • Positive control

    • HeLa cells and Cos cells.
  • General notes

     This product was previously labelled as Nucleoporin p62

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6
    Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    ab188413 was purified from serum-free culture medium of the hybridoma.
  • Clonality

    Monoclonal
  • Clone number

    2A
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Other
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Pore
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Signal Transduction
    • Growth Factors/Hormones
    • EGF
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Other

Images

  • Western blot - Anti-Nucleoporin p62/NUP62 antibody [2A] (ab188413)
    Western blot - Anti-Nucleoporin p62/NUP62 antibody [2A] (ab188413)
    Anti-Nucleoporin p62/NUP62 antibody [2A] (ab188413) at 1/500 dilution + HeLa nuclear membrane lysate

    Secondary
    Alkaline phosphatase-conjugated anti-rat IgG

    Predicted band size: 53 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody [2A] (ab188413)
    Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody [2A] (ab188413)

    Immunofluorescent analysis of HeLa cells labeling Nucleoporin p62/NUP62 with ab188413 at 1/400 dilution, followed by Alexa Fluor®488-conjugated goat anti-rat IgG secondary antibody at 1/500 dilution. HeLa cells were fixed with 3.7% formaldehyde and
    permeabilized with 0.5% Triton X-100.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody [2A] (ab188413)
    Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody [2A] (ab188413)

    Immunofluorescent analysis of HeLa cells, labeling Nucleoporin p62/NUP62 with ab188413 at 1/400 dilution, followed by Alexa Fluor®488-conjugated goat anti-rat IgG secondary antibody at 1/500 dilution. HeLa cells were fixed with 3.7% formaldehyde and
    permeabilized with 0.5% Triton X-100.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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