Anti-TRIM16 antibody (ab72131)
Key features and details
- Rabbit polyclonal to TRIM16
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM16 antibody
See all TRIM16 primary antibodies -
Description
Rabbit polyclonal to TRIM16 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide corresponding to a region between residue 514 and 564 of human TRIM16, NP_006461.3.
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Positive control
- Whole cell lysate from Hela, 293T and NIH3T3 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRIM16 antibody (ab72131)All lanes : Anti-TRIM16 antibody (ab72131) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size: 64 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Various other fainter bands are detected, ranging from 16-250 kDa. -
Immunoprecipitation - Anti-TRIM16 antibody (ab72131)Immunoprecipitation/ Western Blot of TRIM16.
Lane 1: ab72131 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72131 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 3 seconds.
