Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (ab248564)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6402] to PRAS40 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PRAS40 antibody [EPR6402] - BSA and Azide free
    See all PRAS40 primary antibodies

  • Description

    Rabbit monoclonal [EPR6402] to PRAS40 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, HeLa, and MCF-7 whole cell lysates.

  • General notes

    Ab248564 is the carrier-free version of ab133584. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248564 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (ab248564)
    Western blot - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (ab248564)
    All lanes : Anti-PRAS40 antibody [EPR6402] (ab133584) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : AKT1S1 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF-7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 27 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab133584, the same antibody clone in a different buffer formulation.

    Lanes 1 - 4: Merged signal (red and green). Green - ab133584 observed at 40 kDa. Red - loading control, ab130007, observed at 125 kDa.

    ab133584 was shown to specifically react with PRAS40 in wild-type HAP1 cells as signal was lost in AKT1S1 (PRAS40) knockout cells. Wild-type and AKT1S1 (PRAS40) knockout samples were subjected to SDS-PAGE. ab133584 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (ab248564)
    Western blot - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (ab248564)
    All lanes : Anti-PRAS40 antibody [EPR6402] (ab133584) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : L6 cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : RAW 264.7 cell lysate
    Lane 5 : PC12 cell lysate
    Lane 6 : NIH 3T3 cell lysate
    Lane 7 : 293T cell lysate
    Lane 8 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 27 kDa



    This data was developed using ab133584, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (ab248564)
    Western blot - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (ab248564)
    All lanes : Anti-PRAS40 antibody [EPR6402] (ab133584) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : AKT1S1 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF-7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 27 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133584).

    Lanes 1 - 4: Merged signal (red and green). Green - ab133584 observed at 40 kDa. Red - loading control, ab130007, observed at 125 kDa.

    ab133584 was shown to specifically react with PRAS40 in wild-type HAP1 cells as signal was lost in AKT1S1 (PRAS40) knockout cells. Wild-type and AKT1S1 (PRAS40) knockout samples were subjected to SDS-PAGE. Ab133584 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • OI-RD Scanning - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (ab248564)
    OI-RD Scanning - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (ab248564)
    This data was developed using ab133584, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (ab248564)
    Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (ab248564)

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