Porcine VEGF-A ELISA Kit (ab218298)
Key features and details
- Sensitivity: 40 pg/ml
- Range: 40 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Overview
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Product name
Porcine VEGF-A ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 Inter-assay Sample n Mean SD CV% 1 -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
40 pg/ml -
Range
40 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 104.8 97% - 113% Serum 99.39 86% - 112% Plasma 80.15 67% - 104% -
Assay time
4h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Pig -
Product overview
The Porcine VEGF-A ELISA kit (ab218298) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Porcine VEGF-A in serum (Porcine VEGF-A concentration is low in normal serum and plasma samples and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for VEGF-A coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF-A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Porcine VEGF-A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF-A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is Measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X Streptavidin-Conjugated HRP 1 x 200µl 5X Assay Diluent B 1 x 15ml Porcine VEGF-A coated Microplate (12 x 8 wells) 1 x 96 tests Assay Diluent C 1 x 30ml Biotinylated Anti-Pig VEGF-A Detection Antibody 2 vials Lyophilized Pig VEGF-A Standard 2 vials Stop Solution 1 x 8ml TMB Substrate 1 x 12ml -
Research areas
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Relevance
Vascular endothelial growth factor (VEGF) is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. -
Cellular localization
Secreted -
Alternative names
- Vascular endothelial growth factor A
- Vascular permeability factor
- VEGF
see all -
Database links
Images
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Porcine VEGF-A ELISA kit (ab218298) Standard curve.
Porcine VEGF-A ELISA kit (ab218298) Standard curve.