Anti-Mesothelin antibody [SP74] (ab93620)
![Anti-Mesothelin antibody [SP74] (ab93620)](https://www.abcam.com/ps/products/93/ab93620/Images/ab93620-4-anti-mesothelin-antibody-sp74-western-blot-human.jpg "Anti-Mesothelin antibody [SP74] (ab93620)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP74] to Mesothelin
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Mesothelin antibody [SP74]
See all Mesothelin primary antibodies -
Description
Rabbit monoclonal [SP74] to Mesothelin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Mesothelin aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q13421 -
Positive control
- IHC-P: Human Mesothelioma tissue. WB: BxPC-3, HeLa, PC3 and A549 cell lysate.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP74 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Mesothelin antibody [SP74] (ab93620)All lanes : Anti-Mesothelin antibody [SP74] (ab93620) at 0.322 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate
Lane 2 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) cell lysate
Lane 3 : PC3 (Human prostate adenocarcinoma epithelial cell) cell lysate
Lane 4 : A549 (Human lung carcinoma epithelial cell) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 69 kDa
Observed band size: 49,69 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer and concentration: 5% NFDM /TBST -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620)](https://www.abcam.com/ps/products/93/ab93620/Images/ab93620-3-AntiMesothelin-antibody-SP74-IHC-Human-mesothelioma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human mesothelioma tissue sections labeling Mesothelin with Purified ab93620 at 1/100 dilution (1.4 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620)](https://www.abcam.com/ps/products/93/ab93620/Images/ab93620-281675-anti-mesothelin-antibody-sp74-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620)Human Mesothelioma stained with Anti-Mesothelin antibody [SP74] (ab93620)
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![Anti-Mesothelin antibody [SP74] (ab93620)](https://www.abcam.com/ps/products/93/ab93620/Images/ab93620-5-benefits-of-recombinant-antibodies.png)
Anti-Mesothelin antibody [SP74] (ab93620)
