Human CCL18 Matched Antibody Pair Kit (ab219527)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 3.1 pg/ml - 200 pg/ml
Overview
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Product name
Human CCL18 Matched Antibody Pair Kit
See all CCL18 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
1.4 pg/ml -
Range
3.1 pg/ml - 200 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human CCL18 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human CCL18.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab211649. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human CCL18 Capture Antibody 2 x 50µg 1 x 50µg Human CCL18 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human CCL18 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. -
Tissue specificity
Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Alternative macrophage activation associated CC chemokine 1
- Alternative macrophage activation-associated CC chemokine 1
- AMAC-1
see all -
Database links
- Entrez Gene: 6362 Human
- Omim: 603757 Human
- SwissProt: P55774 Human
- Unigene: 143961 Human
Images
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Human MIP4 standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human CCL18 Matched Antibody Pair Kit (ab219527)To learn more about the advantages of recombinant antibodies see here.
