Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VSIG4 antibody [EPR22576-125] - BSA and Azide free (ab255690)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22576-125] to VSIG4 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-VSIG4 antibody [EPR22576-125] - BSA and Azide free
    See all VSIG4 primary antibodies

  • Description

    Rabbit monoclonal [EPR22576-125] to VSIG4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-Fr

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human liver and placenta tissue lysate; Jurkat and THP-1 cell lysate; His-tagged human VSIG4 recombinant protein (aa 19-232). IHC-P: Human liver, placenta and lung tissue. IP: Human placenta lysate.

  • General notes

    Ab255690 is the carrier-free version of ab252932. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255690 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-125] - BSA and Azide free (ab255690)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-125] - BSA and Azide free (ab255690)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling VSIG4 with ab252932 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells of human liver (PMID: 17016562) is observed. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using ab208572 retrieval buffer.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252932).

  • Immunoprecipitation - Anti-VSIG4 antibody [EPR22576-125] - BSA and Azide free (ab255690)
    Immunoprecipitation - Anti-VSIG4 antibody [EPR22576-125] - BSA and Azide free (ab255690)

    VSIG4 was immunoprecipitated from 0.35 mg human placenta lysate with ab252932 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab252932 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/1000 dilution.

    Lane 1: Human placenta lysate 10μg (Input).
    Lane 2: ab252932 IP in Human placenta lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252932 in human placenta lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    The molecular weight observed is consistent with what has been described in previous literatures (PMID: 16009265, 17016562).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252932).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-125] - BSA and Azide free (ab255690)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-125] - BSA and Azide free (ab255690)

    Immunohistochemical analysis of paraffin-embedded human lung tissue labeling VSIG4 with ab252932 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on macrophages of human lung (PMID: 17016562) is observed. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using ab208572 retrieval buffer.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252932).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-125] - BSA and Azide free (ab255690)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-125] - BSA and Azide free (ab255690)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling VSIG4 with ab252932 at 1/100 dilution  followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stroma of human placenta (PMID: 17016562) is observed. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using ab208572 retrieval buffer.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252932).

  • Anti-VSIG4 antibody [EPR22576-125] - BSA and Azide free (ab255690)
    Anti-VSIG4 antibody [EPR22576-125] - BSA and Azide free (ab255690)

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