Anti-VSIG4 antibody [EPR22576-125] (ab252932)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22576-125] to VSIG4
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-VSIG4 antibody [EPR22576-125]
See all VSIG4 primary antibodies -
Description
Rabbit monoclonal [EPR22576-125] to VSIG4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human liver and placenta tissue lysate; Jurkat and THP-1 cell lysate; His-tagged human VSIG4 recombinant protein (aa 19-232). IHC-P: Human liver, placenta and lung tissue. IP: Human placenta lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22576-125 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-125] (ab252932)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling VSIG4 with ab252932 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells of human liver (PMID: 17016562) is observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using ab208572 retrieval buffer.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .
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VSIG4 was immunoprecipitated from 0.35 mg human placenta lysate with ab252932 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab252932 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/1000 dilution.
Lane 1: Human placenta lysate 10μg (Input).
Lane 2: ab252932 IP in Human placenta lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252932 in human placenta lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
The molecular weight observed is consistent with what has been described in previous literatures (PMID: 16009265, 17016562).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-125] (ab252932)
Immunohistochemical analysis of paraffin-embedded human lung tissue labeling VSIG4 with ab252932 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on macrophages of human lung (PMID: 17016562) is observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using ab208572 retrieval buffer.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-125] (ab252932)
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling VSIG4 with ab252932 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stroma of human placenta (PMID: 17016562) is observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using ab208572 retrieval buffer.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .
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All lanes : Anti-VSIG4 antibody [EPR22576-125] (ab252932) at 1/1000 dilution
Lane 1 : Human liver tissue lysate
Lane 2 : Human placenta tissue lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood), whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 2-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 37,50 kDa why is the actual band size different from the predicted?Blocking/diluting buffer and concentration: 5% NFDM/TBST.
Exposure time:
Lane 1: 125 seconds
Lane 2: 3 minutes
Lanes 3-4: 125 seconds
The molecular weight observed is consistent with what has been described in previous literatures (PMID: 16009265, 17016562)
The expression profile observed is consistent with the literatures (PMID: 16009265, 17016562)
Negative control: Jurkat (PMID: 16009265)
Lanes 1, 3 and 4 were developed using a higher sensitivity ECL substrate.
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Anti-VSIG4 antibody [EPR22576-125] (ab252932) at 1/1000 dilution + His-tagged human VSIG4 recombinant protein (aa 19-232) 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Blocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes.
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