Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22576-70] to VSIG4 - BSA and Azide free
  • Suitable for: IP, IHC-Fr, WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free
    See all VSIG4 primary antibodies

  • Description

    Rabbit monoclonal [EPR22576-70] to VSIG4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-Fr, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human liver and placenta tissue lysate; Jurkat and THP-1 cell lysate; Mouse liver tissue lysate.His-tagged human VSIG4 recombinant protein (aa 19-232). IHC-P: Human liver, placenta and lung tissue; Mouse lung tissue. IHC-Fr: Mouse liver tissue. IP: Human placenta lysate.

  • General notes

    Ab255692 is the carrier-free version of ab252933. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255692 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)
    Immunohistochemistry (Frozen sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling ab252933 with VSIG4 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000) dilution. Positive staining on Kupffer cells of mouse liver (PMID: 17016562) is observed. The nuclear counterstain is DAPI (Blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252933).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells of human liver (PMID: 17016562) is observed. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using ab208572 retrieval buffer.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252933).

  • Immunoprecipitation - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)
    Immunoprecipitation - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)

    VSIG4 was immunoprecipitated from 0.35 mg of human placenta lysate with ab252933 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab252933 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

    Lane 1: Human placenta lysate 10 μg (Input). 
    Lane 2: ab252933 IP in human placenta lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252933 in human placenta lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    The molecular weight observed is consistent with what has been described in previous literature (PMID: 16009265, 17016562).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252933).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)

    Immunohistochemical analysis of paraffin-embedded human lung tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on macrophages of human lung (PMID: 17016562) is observed. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using ab208572 retrieval buffer.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252933).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stroma of human placenta (PMID: 17016562) is observed. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using ab208572 retrieval buffer.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252933).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells of mouse liver (PMID: 17016562) is observed. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using ab208572 retrieval buffer.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252933).

  • Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)
    Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)

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