Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CXCL7/PBP antibody [EPR20036] (ab206406)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20036] to CXCL7/PBP
  • Suitable for: IHC-P, WB, IHC-Fr, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CXCL7/PBP antibody [EPR20036]
    See all CXCL7/PBP primary antibodies

  • Description

    Rabbit monoclonal [EPR20036] to CXCL7/PBP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse CXCL7/PBP active protein; Mouse spleen, platelet, plasma and serum lysates. IHC-P: Mouse spleen and lung tissues. IHC-Fr: Mouse spleen tissue. IP: Mouse spleen lysate.

  • General notes

    Previously labelled as CXCL7. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)
    Western blot - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)
    Anti-CXCL7/PBP antibody [EPR20036] (ab206406) at 1/5000 dilution + Mouse CXCL7/PBP active protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa
    Observed band size: 7 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Frozen sections) - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)
    Immunohistochemistry (Frozen sections) - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling CXCL7/PBP with ab206406 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on megakaryocytes and platelets of mouse spleen was observed. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CXCL7/PBP with ab206406 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on megakaryocytes and platelets of mouse spleen is observed [PMID:16391012]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)
    Western blot - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)
    Lanes 1-2 : Anti-CXCL7/PBP antibody [EPR20036] (ab206406) at 1/2000 dilution
    Lanes 3-4 : Anti-CXCL7/PBP antibody [EPR20036] (ab206406) at 1/10000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Mouse plasma
    Lane 3 : Mouse platelet lysate
    Lane 4 : Mouse serum

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 14 kDa
    Observed band size: 7 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1/2: 4 seconds; Lane 3/4: 1 second.

    The molecular weight is consistent with what has been described in the literature: PMID: 14673015.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CXCL7/PBP with ab206406 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on platelets of mouse lung is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)
    Immunoprecipitation - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)

    CXCL7/PBP was immunoprecipitated from 0.35 mg of Mouse spleen lysate with ab206406 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206406 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse spleen lysate, 10 μg (Input).

    Lane 2: ab206406 IP in Mouse spleen lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206406 in Mouse spleen lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-CXCL7/PBP antibody [EPR20036] (ab206406)
    Anti-CXCL7/PBP antibody [EPR20036] (ab206406)

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