Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

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Anti-CXCL7/PBP antibody [EPR20036] (ab206406)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20036] to CXCL7/PBP
Suitable for: IHC-P, WB, IHC-Fr, IP
Reacts with: Mouse

Product name

Anti-CXCL7/PBP antibody [EPR20036]
See all CXCL7/PBP primary antibodies
Description

Rabbit monoclonal [EPR20036] to CXCL7/PBP
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse CXCL7/PBP active protein; Mouse spleen, platelet, plasma and serum lysates. IHC-P: Mouse spleen and lung tissues. IHC-Fr: Mouse spleen tissue. IP: Mouse spleen lysate.
General notes
Previously labelled as CXCL7.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20036
Isotype

IgG
Research areas

Western blot - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)

Anti-CXCL7/PBP antibody [EPR20036] (ab206406) at 1/5000 dilution + Mouse CXCL7/PBP active protein at 0.01 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 14 kDa
Observed band size: 7 kDa
why is the actual band size different from the predicted?

Exposure time: 8 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Frozen sections) - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling CXCL7/PBP with ab206406 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on megakaryocytes and platelets of mouse spleen was observed. The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CXCL7/PBP with ab206406 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on megakaryocytes and platelets of mouse spleen is observed [PMID:16391012]. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)

Lanes 1-2 : Anti-CXCL7/PBP antibody [EPR20036] (ab206406) at 1/2000 dilution
Lanes 3-4 : Anti-CXCL7/PBP antibody [EPR20036] (ab206406) at 1/10000 dilution

Lane 1 : Mouse spleen lysate
Lane 2 : Mouse plasma
Lane 3 : Mouse platelet lysate
Lane 4 : Mouse serum

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 14 kDa
Observed band size: 7 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1/2: 4 seconds; Lane 3/4: 1 second.

The molecular weight is consistent with what has been described in the literature: PMID: 14673015.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CXCL7/PBP with ab206406 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on platelets of mouse lung is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-CXCL7/PBP antibody [EPR20036] (ab206406)

CXCL7/PBP was immunoprecipitated from 0.35 mg of Mouse spleen lysate with ab206406 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206406 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Mouse spleen lysate, 10 μg (Input).

Lane 2: ab206406 IP in Mouse spleen lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206406 in Mouse spleen lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Anti-CXCL7/PBP antibody [EPR20036] (ab206406)

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