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Immunology Innate Immunity Macrophage / Inflamm.

Anti-CXCL7/PBP antibody [EPR19780] (ab206966)

Price and availability

298 185 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-CXCL7/PBP antibody [EPR19780] (ab206966)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19780] to CXCL7/PBP
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-CXCL7/PBP antibody [EPR19780]
    See all CXCL7/PBP primary antibodies
  • Description

    Rabbit monoclonal [EPR19780] to CXCL7/PBP
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human NAP2 active recombinant protein; human serum and human spleen lysate. IP: Human serum.
  • General notes

     

     

    Previously labelled as CXCL7. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19780
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Platelets
    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)
    • Signal Transduction
    • Growth Factors/Hormones
    • PDGF

Images

  • Western blot - Anti-CXCL7/PBP antibody [EPR19780] (ab206966)
    Western blot - Anti-CXCL7/PBP antibody [EPR19780] (ab206966)
    Anti-CXCL7/PBP antibody [EPR19780] (ab206966) at 1/1000 dilution + Human NAP2 active recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa
    Observed band size: 11 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds
  • Western blot - Anti-CXCL7/PBP antibody [EPR19780] (ab206966)
    Western blot - Anti-CXCL7/PBP antibody [EPR19780] (ab206966)
    All lanes : Anti-CXCL7/PBP antibody [EPR19780] (ab206966) at 1/1000 dilution

    Lane 1 : Human serum at 20 µg
    Lane 2 : Human spleen lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 14 kDa
    Observed band size: 11 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 10 seconds; Lane 2: 3 minutes.

  • Immunoprecipitation - Anti-CXCL7/PBP antibody [EPR19780] (ab206966)
    Immunoprecipitation - Anti-CXCL7/PBP antibody [EPR19780] (ab206966)

    NAP2 was immunoprecipitated from 0.35 mg of Human serum with ab206966 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab206966 at 1/500 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human serum 10µg (Input).

    Lane 2: ab206966 IP in Human serum. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206966 in Human serum.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    The band at 50 kD is likely to be human IgG in input and rabbit IgG heavy chain in +ve and –ve lane, but this has not been tested.

  • Anti-CXCL7/PBP antibody [EPR19780] (ab206966)
    Anti-CXCL7/PBP antibody [EPR19780] (ab206966)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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