Anti-CXCL7/PBP antibody [EPR19780] (ab206966)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19780] to CXCL7/PBP
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-CXCL7/PBP antibody [EPR19780]
See all CXCL7/PBP primary antibodies -
Description
Rabbit monoclonal [EPR19780] to CXCL7/PBP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human NAP2 active recombinant protein; human serum and human spleen lysate. IP: Human serum.
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General notes
Previously labelled as CXCL7.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19780 -
Isotype
IgG -
Research areas
Images
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Anti-CXCL7/PBP antibody [EPR19780] (ab206966) at 1/1000 dilution + Human NAP2 active recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
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All lanes : Anti-CXCL7/PBP antibody [EPR19780] (ab206966) at 1/1000 dilution
Lane 1 : Human serum at 20 µg
Lane 2 : Human spleen lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 14 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 10 seconds; Lane 2: 3 minutes.
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NAP2 was immunoprecipitated from 0.35 mg of Human serum with ab206966 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab206966 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Human serum 10µg (Input).
Lane 2: ab206966 IP in Human serum.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206966 in Human serum.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
The band at 50 kD is likely to be human IgG in input and rabbit IgG heavy chain in +ve and –ve lane, but this has not been tested.
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