Anti-CSF-1-R antibody [EPR21885-161] (ab221684)
![Anti-CSF-1-R antibody [EPR21885-161] (ab221684)](https://www.abcam.com/ps/products/221/ab221684/Images/ab221684-308550-anti-mcsf-receptor-antibody-epr21885-161-western-blot.jpg "Anti-CSF-1-R antibody [EPR21885-161] (ab221684)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21885-161] to CSF-1-R
- Suitable for: WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-CSF-1-R antibody [EPR21885-161]
See all CSF-1-R primary antibodies -
Description
Rabbit monoclonal [EPR21885-161] to CSF-1-R -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat lung lysate, Mouse spleen and placenta lysate, J774A.1 and RAW 264.7 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21885-161 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CSF-1-R antibody [EPR21885-161] (ab221684)All lanes : Anti-CSF-1-R antibody [EPR21885-161] (ab221684) at 1/1000 dilution
Lane 1 : Rat lung lysate at 20 µg
Lane 2 : Mouse spleen lysate at 20 µg
Lane 3 : Mouse placenta lysate at 20 µg
Lane 4 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 10 µg
Lane 5 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution
Predicted band size: 108 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-4: 3 minutes; Lane 5: 32 seconds.
The molecular mass observed is consistent with the literature (PMID 21610095; PMID 20436485; PMID 24362524; PMID: 26066800).
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![Anti-CSF-1-R antibody [EPR21885-161] (ab221684)](https://www.abcam.com/ps/products/221/ab221684/Images/ab221684-2-benefits-of-recombinant-antibodies.png)
Anti-CSF-1-R antibody [EPR21885-161] (ab221684)
