Anti-PFKFB3 (phospho S461) antibody [EPR19735] (ab202291)
![Anti-PFKFB3 (phospho S461) antibody [EPR19735] (ab202291)](https://www.abcam.com/ps/products/202/ab202291/Images/ab202291-277769-anti-pfkfb3-phospho-s461-antibody-epr19735-western-blot.jpg "Anti-PFKFB3 (phospho S461) antibody [EPR19735] (ab202291)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19735] to PFKFB3 (phospho S461)
- Suitable for: Dot blot, WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-PFKFB3 (phospho S461) antibody [EPR19735]
See all PFKFB3 primary antibodies -
Description
Rabbit monoclonal [EPR19735] to PFKFB3 (phospho S461) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with mPFKFB3 (WT) whole cell lysate. ICC/IF: Flag-mPFKFB3 transfected HEK-293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19735 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PFKFB3 (phospho S461) antibody [EPR19735] (ab202291)All lanes : Anti-PFKFB3 (phospho S461) antibody [EPR19735] (ab202291) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with empty vector (vector control), whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with mPFKFB3 (WT) whole cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with mPFKFB3 (WT), then treated with alkaline phosphatase for 1 hour, whole cell lysate
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with mPFKFB3 S461A mutant, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 2% BSA/TBST.
The plasmids were kindly provided by our collaborator Dr. Jianghuai Liu, Nanjing University MARC.
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![Dot Blot - Anti-PFKFB3 (phospho S461) antibody [EPR19735] (ab202291)](https://www.abcam.com/ps/products/202/ab202291/Images/ab202291-277770-anti-pfkfb3-phospho-s461-antibody-epr19735-dot-blot.jpg)
Dot Blot - Anti-PFKFB3 (phospho S461) antibody [EPR19735] (ab202291)Dot blot analysis of PFKFB3 (phospho S461) labeled with ab202291 at 1/1000 dilution.
Lane 1: PFKFB3 (phospho S461) peptide;
Lane 2: PFKFB3 non-phospho peptide.Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Immunocytochemistry/ Immunofluorescence - Anti-PFKFB3 (phospho S461) antibody [EPR19735] (ab202291)](https://www.abcam.com/ps/products/202/ab202291/Images/ab202291-277767-anti-pfkfb3-phospho-s461-antibody-epr19735-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PFKFB3 (phospho S461) antibody [EPR19735] (ab202291)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney) cells labeling PFKFB3 (phospho S461) with ab202291 at 1/100 dilution. Counterstained with Anti-FLAG antibody (green).
The results showed positive staining on Flag-mPFKFB3 transfected HEK-293T cells and no staining on Flag-mPFKFB3 S461A mutant transfected HEK-293T cells. The image was provided by our collaborator Dr. Jianghuai Liu, Nanjing University MARC.
The nuclear counter stain is DAPI (blue).
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![Anti-PFKFB3 (phospho S461) antibody [EPR19735] (ab202291)](https://www.abcam.com/ps/products/202/ab202291/Images/ab202291-5-benefits-of-recombinant-antibodies.png)
Anti-PFKFB3 (phospho S461) antibody [EPR19735] (ab202291)
