Anti-PFKFB3 (phospho S461) antibody [EPR19735] - BSA and Azide free (ab232498)
![Anti-PFKFB3 (phospho S461) antibody [EPR19735] - BSA and Azide free (ab232498)](https://www.abcam.com/ps/products/232/ab232498/Images/ab232498-2-ab202291277770202291DB.jpg "Anti-PFKFB3 (phospho S461) antibody [EPR19735] - BSA and Azide free (ab232498)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19735] to PFKFB3 (phospho S461) - BSA and Azide free
- Suitable for: Dot blot, WB, ICC/IF
- Reacts with: Mouse
Overview
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Product name
Anti-PFKFB3 (phospho S461) antibody [EPR19735] - BSA and Azide free
See all PFKFB3 primary antibodies -
Description
Rabbit monoclonal [EPR19735] to PFKFB3 (phospho S461) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with mPFKFB3 (WT) whole cell lysate. ICC/IF: Flag-mPFKFB3 transfected HEK-293T cells.
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General notes
Ab232498 is the carrier-free version of ab202291. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab232498 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19735 -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-PFKFB3 (phospho S461) antibody [EPR19735] - BSA and Azide free (ab232498)
Dot blot analysis of PFKFB3 (phospho S461) labeled with ab202291 at 1/1000 dilution.
Lane 1: PFKFB3 (phospho S461) peptide;
Lane 2: PFKFB3 non-phospho peptide.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202291).
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![Immunocytochemistry/ Immunofluorescence - Anti-PFKFB3 (phospho S461) antibody [EPR19735] - BSA and Azide free (ab232498)](https://www.abcam.com/ps/products/232/ab232498/Images/ab232498-309037-291.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PFKFB3 (phospho S461) antibody [EPR19735] - BSA and Azide free (ab232498)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney) cells labeling PFKFB3 (phospho S461) with ab202291 at 1/100 dilution. Counterstained with Anti-FLAG antibody (green).
The results showed positive staining on Flag-mPFKFB3 transfected HEK-293T cells and no staining on Flag-mPFKFB3 S461A mutant transfected HEK-293T cells. The image was provided by our collaborator Dr. Jianghuai Liu, Nanjing University MARC.
The nuclear counter stain is DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202291).
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![Anti-PFKFB3 (phospho S461) antibody [EPR19735] - BSA and Azide free (ab232498)](https://www.abcam.com/ps/products/232/ab232498/Images/ab232498-3-benefits-of-recombinant-antibodies.png)
Anti-PFKFB3 (phospho S461) antibody [EPR19735] - BSA and Azide free (ab232498)
