Astana Biomed Group, an authorized Abcam distributor in Central Asia

Alexa Fluor® 488 Anti-SIRT1 antibody [19A7AB4] - Nuclear Marker (ab157401)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [19A7AB4] to SIRT1 - Nuclear Marker
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG1

Overview

  • Product name

    Alexa Fluor® 488 Anti-SIRT1 antibody [19A7AB4] - Nuclear Marker
    See all SIRT1 primary antibodies

  • Description

    Alexa Fluor® 488 Mouse monoclonal [19A7AB4] to SIRT1 - Nuclear Marker

  • Host species

    Mouse

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: RatDoes not react with: Cow

  • Immunogen

    Recombinant full length protein corresponding to Human SIRT1.

  • Positive control

    • HeLa and HFDn cells.

  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SIRT1 antibody [19A7AB4] - Nuclear Marker (ab157401)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SIRT1 antibody [19A7AB4] - Nuclear Marker (ab157401)

    ab157401 staining SIRT1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab157401 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SIRT1 antibody [19A7AB4] - Nuclear Marker (ab157401)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SIRT1 antibody [19A7AB4] - Nuclear Marker (ab157401)
    Immunocytochemical analysis: A) HeLa cells (40x) stained using ab157401 at 1.0 µg/mL as green; anti-HSP60 (1/3000, ab46798) as red, and DAPI in blue, as a nuclear stain. Secondary antibody used against ab46798 was goat anti-rabbit Alexa594 (1/2000). B) ab157401, 1.0 µg/mL as green channel only. All blocking and antibodies were diluted in 10% normal goat serum.
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SIRT1 antibody [19A7AB4] - Nuclear Marker (ab157401)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SIRT1 antibody [19A7AB4] - Nuclear Marker (ab157401)
    Immunocytochemical analysis: A)HFDn cells (40x) stained using ab157401, 1.0 µg/mL as green; anti-HSP60 (1/3000, ab46798) as red, and DAPI in blue, as a nuclear stain. Secondary antibody against ab46798 was goat anti-rabbit Alexa594 (1/2000). B) ab157401, 1.0 µg/mL as green channel only. All blocking and antibodies were diluted in 10% normal goat serum.
  • Flow Cytometry - Alexa Fluor® 488 Anti-SIRT1 antibody [19A7AB4] - Nuclear Marker (ab157401)
    Flow Cytometry - Alexa Fluor® 488 Anti-SIRT1 antibody [19A7AB4] - Nuclear Marker (ab157401)
    Flow Cytometryic analysis of HeLa cells labeling SIRT1 with ab157401 at 0.2 µg/mL dilution (red). Isotype control antibody at 0.2 µg/mL dilution (black).

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