Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-SIRT1 (phospho S47) antibody [EPR2849Y] (ab76039)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2849Y] to SIRT1 (phospho S47)
Suitable for: WB, IHC-P, ICC/IF, Dot blot
Reacts with: Human

Product name

Anti-SIRT1 (phospho S47) antibody [EPR2849Y]
See all SIRT1 primary antibodies
Description

Rabbit monoclonal [EPR2849Y] to SIRT1 (phospho S47)
Host species

Rabbit
Specificity

This antibody detects SIRT1 phosphorylated on serine 47

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

A phospho specific peptide corresponding to residues surrounding serine 47 of human SIRT1
Positive control
- 293 cell lysate IHC-P: human gastric adenocarcinoma tissue
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2849Y
Isotype

IgG
Research areas

Western blot - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] (ab76039)

All lanes : Anti-SIRT1 (phospho S47) antibody [EPR2849Y] (ab76039) at 1/2000 dilution

Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 2 : A549 treated with 1mM AICAR for 48 hours whole cell lysate
Lane 3 : A549 treated with 1mM AICAR for 48 hours whole cell lysate. Then the membrane was incubated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 82 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] (ab76039)

Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue sections labeling SIRT1 with purified ab76039 at 1/300 dilution. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin.
Antigen retrieval was heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) .
The immunostaining was performed on a Leica Biosystems BOND™ RX instrument
Immunocytochemistry/ Immunofluorescence - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] (ab76039)

Immunocytochemistry/Immunofluorescence analysis of A549 +/- LP cells labelling SIRT1 (phospho S47) with ab76039 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 (goat anti-rabbit IgG Alexa Fluor^® 488) (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at a 1/200 dilution. Nuclei counterstained with DAPI (blue).
Dot Blot - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] (ab76039)

Dot blot analysis of SIRT1 (phospho S47) phospho peptide (Lane 1) and SIRT1 non-phospho peptide (Lane 2) labelling SIRT1 (phospho S47) with ab76039 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
Western blot - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] (ab76039)

All lanes : Anti-SIRT1 (phospho S47) antibody [EPR2849Y] (ab76039) at 1/1000 dilution

Lane 1 : NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : NIH:OVCAR-3 treated with 2mM H2O2 for 30 minutes whole cell lysate
Lane 3 : NIH:OVCAR-3 treated with 2mM H2O2 for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

Predicted band size: 82 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] (ab76039)

All lanes : Anti-SIRT1 (phospho S47) antibody [EPR2849Y] (ab76039) at 1/2000 dilution

Lane 1 : 293 cell lysate
Lane 2 : 293 cell lysate treated with LP

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 82 kDa
Observed band size: 82 kDa
Anti-SIRT1 (phospho S47) antibody [EPR2849Y] (ab76039)

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