Anti-SIRT1 antibody (ab263965)
Key features and details
- Rabbit polyclonal to SIRT1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SIRT1 antibody
See all SIRT1 primary antibodies -
Description
Rabbit polyclonal to SIRT1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human SIRT1 (C terminal).
Database link: Q96EB6 -
Positive control
- WB: HCT116, K562, Jurkat, A549 and LNCap whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SIRT1 antibody (ab263965)All lanes : Anti-SIRT1 antibody (ab263965) at 1/1000 dilution
Lane 1 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 5 : LNCaP (human prostate cancer cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Rabbit secondary HRP antibody at 1/70000 dilution
Predicted band size: 81 kDaPrimary incubation overnight at 4°C.
Secondary incubation for 45 minutes at RT.
