Canine TNF alpha ELISA Kit (ab193687)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 2 pg/ml - 700 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine TNF alpha ELISA Kit
See all TNF alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2 pg/ml -
Range
2 pg/ml - 700 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 101.1 93% - 109% Plasma 97.84 91% - 104% Cell culture media 118.4 110% - 126% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Abcam’s TNF alpha Canine ELISA Kit (ab193687) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of canine TNF alpha in serum, plasma and cell culture supernatant.
This assay employs an antibody specific for canine TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures TNF alpha present in the samples. The wells are washed and biotinylated anti-canine TNF alpha antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of TNF alpha bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 350X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated Canine TNF-alpha detection Antibody (lyophilized) 2 vials Canine TNF-alpha Standards (lyophilized) 2 vials Pre coated canine TNF-alpha Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
Involvement in disease
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- APC1
- APC1 protein
- Cachectin
see all -
Database links
Images
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Standard Curve.
Standard Curve in Assay Diluent A using ab193687 TNF alpha Canine ELISA Kit.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Standard Curve.Standard Curve in Assay Diluent B using ab193687 TNF alpha Canine ELISA Kit.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.