Recombinant human ABL1 (mutated Y253F) protein (ab140419)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
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Product name
Recombinant human ABL1 (mutated Y253F) protein
See all ABL1 proteins and peptides -
Biological activity
The specific activity of ab140419 was determined to be 52 nmol/min/mg. -
Purity
> 80 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
EALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASG DNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHS WYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRI NTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVY GVSPNYDKWEMERTDITMKHKLGGGQFGEVYEGVWKKYSLTVAVKTLKED TMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDY LRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHL VKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFG VLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACW QWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAP ELPTKTRTSRRAAEHRDTTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKE RGPPEGGLNEDERLLPKDKKTNLFSALIKKKKKTAPTPPKRSSSFREMDG QPERRGAGEEEGRDISNGALAFTPLDTADPAKSPKPSNGAGVPNGALRES GGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSASCVPHGA KDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVT RGTVTPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAP ASGLPHKEEAGKGSALGTPAAAEPVTPTSKAGSGAPGGTSKGPAEESRVR RHKHSSESPGRDKGKLSRLKPAPPPPPAASAGKAGGKPSQSPSQEAAGEA VLGAKTKATSLVDAVNSDAAKPSQPGEGLKKPVLPATPKPQSAKPSGTPI SPAPVPSTLPSASSALAGDQPSSTAFIPLISTRVSLRKTRQPPERIASGA ITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQ MRNKFAFREAINKLENNLRELQICPATAGSGPAATQDFSKLLSSVKEISD IVQR -
Predicted molecular weight
160 kDa including tags -
Amino acids
27 to 1130
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Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human ABL1 (mutated Y253F) protein (ab140419)Kinase Activity Plot. The specific activity of ab140419 was determined to be 52 nmol/min/mg.
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SDS-PAGE - Recombinant human ABL1 (mutated Y253F) protein (ab140419)SDS-PAGE analysis of ab140419.
