Recombinant human ABL1 (mutated T315I) protein (Active) (ab196134)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human ABL1 (mutated T315I) protein (Active)
See all ABL1 proteins and peptides -
Biological activity
Specific Activity: 222 pmoles/min/μg.
Assay Conditions: Activity was determined using a 100% phosphorylated Tyr02 peptide and a Z-lyte kinase assay. Reaction was incubated at 30°C for 1 hour. -
Purity
> 75 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
LGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPN LVQLLGVCTREPPFYIIIEFMTYGNLLDYLRECNRQEVNAVVLLYMATQI SSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHA GAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQ VYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMF QESSISDEVEKELGKQGVRGAVSTLLQAPELPTK -
Predicted molecular weight
33 kDa including tags -
Amino acids
248 to 531 -
Modifications
mutated T315I -
Tags
His tag N-Terminus -
Additional sequence information
GenBank Accession No. NM_005157. Note: This mutation is often cited as T344I in variant B (GenBank Accession No. NM_007313).
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.4% Tris HCl, 0.58% Sodium chloride, 0.05% Tween, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human ABL1 (mutated T315I) protein (Active) (ab196134)
Specific activity of ab196134 was determined to be 222 pmol/min/μg
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SDS-PAGE - Recombinant human ABL1 (mutated T315I) protein (Active) (ab196134)10% SDS-PAGE analysis of ab196134 stained with Coomassie Blue.
Lane 1: ab196134 (4µg)
Lane 2: Protein Marker
