Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 10, 2021

Anti-PON2 antibody [EPR15295-58] (ab183718)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15295-58] to PON2
Suitable for: WB, ICC/IF, IP
Reacts with: Human

Product name

Anti-PON2 antibody [EPR15295-58]
See all PON2 primary antibodies
Description

Rabbit monoclonal [EPR15295-58] to PON2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, U87-MG and HepG2 whole cell lysate. HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15295-58
Isotype

IgG
Research areas

Western blot - Anti-PON2 antibody [EPR15295-58] (ab183718)

All lanes : Anti-PON2 antibody [EPR15295-58] (ab183718) at 1/1000 dilution

Lane 1 : HeLa whole cell lysate
Lane 2 : U87-MG whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa

Blocking/ Dilution buffer: 5% NFDM /TBST.
Western blot - Anti-PON2 antibody [EPR15295-58] (ab183718)

Anti-PON2 antibody [EPR15295-58] (ab183718) at 1/5000 dilution + HepG2 whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa

Blocking/ Dilution buffer: 5% NFDM /TBST.
Immunocytochemistry/ Immunofluorescence - Anti-PON2 antibody [EPR15295-58] (ab183718)

Immunofluorescent analysis of acetone fixed HeLa cells labeling PON2 using ab183718 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.
Immunoprecipitation - Anti-PON2 antibody [EPR15295-58] (ab183718)

Lysate from HepG2 cells was immunoprecipitated with ab183718 at a 1/50 dilution. For the subsequent blot, ab183718 was used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST. Lane 2: TBS instead of HepG2 lysates.
Anti-PON2 antibody [EPR15295-58] (ab183718)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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