Anti-VEGF Receptor 2 antibody [#4 (20I6)] (ab39378)
![Anti-VEGF Receptor 2 antibody [#4 (20I6)] (ab39378)](https://www.abcam.com/ps/products/39/ab39378/Images/ab39378-316618-anti-vegf-receptor-2-antibody-4-20i6-western-blot.jpg "Anti-VEGF Receptor 2 antibody [#4 (20I6)] (ab39378)")
Key features and details
- Mouse monoclonal [#4 (20I6)] to VEGF Receptor 2
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-VEGF Receptor 2 antibody [#4 (20I6)]
See all VEGF Receptor 2 primary antibodies -
Description
Mouse monoclonal [#4 (20I6)] to VEGF Receptor 2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human VEGF Receptor 2 aa 20-678.
Sequence:ASVGLPSVSLDLPRLSIQKDILTIKANTTLQITCRGQRDLDWLWPNNQSG SEQRVEVTECSDGLFCKTLTIPKVIGNDTGAYKCFYRETDLASVIYVYVQ DYRSPFIASVSDQHGVVYITENKNKTVVIPCLGSISNLNVSLCARYPEKR FVPDGNRISWDSKKGFTIPSYMISYAGMVFCEAKINDESYQSIMYIVVVV GYRIYDVVLSPSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPSSKHQH KKLVNRDLKTQSGSEMKKFLSTLTIDGVTRSDQGLYTCAASSGLMTKKNS TFVRVHEKPFVAFGSGMESLVEATVGERVRIPAKYLGYPPPEIKWYKNGI PLESNHTIKAGHVLTIMEVSERDTGNYTVILTNPISKEKQSHVVSLVVYV PPQIGEKSLISPVDSYQYGTTQTLTCTVYAIPPPHHIHWYWQLEEECANE PSQAVSVTNPYPCEEWRSVEDFQGGNKIEVNKNQFALIEGKNKTVSTLVI QAANVSALYKCEAVNKVGRGERVISFHVTRGPEITLQPDMQPTEQESVSL WCTADRSTFENLTWYKLGPQPLPIHVGELPTPVCKNLDTLWKLNATMFSN STNDILIMELKNASLQDQGDYVCLAQDRKTKKRHCVVRQLTVLGRETILD HCAEAVGMP
Database link: P35968-2 -
Positive control
- WB: Recombinant human VEGF Receptor 2 protein. ICC/IF: HUVEC cells. Flow Cyt: HDLEC cells.
Properties
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Form
Lyophilized:Centrifuge vial prior to opening. Please reconstitute this product to 0.1-1.0 mg/mL in sterile water. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). The lyophilized protein is stable for a few weeks at room temperature. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
#4 (20I6) -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-VEGF Receptor 2 antibody [#4 (20I6)] (ab39378)All lanes : Anti-VEGF Receptor 2 antibody [#4 (20I6)] (ab39378) at 5 µg/ml
Lane 1 : Recombinant human VEGF Receptor 2 protein
Lane 2 : Recombinant human VEGF Receptor 2 (aa 20-678) protein
Lane 3 : Recombinant human VEGF Receptor 2 (aa 20-678) protein-Fc
Predicted band size: 110 kDa10% SDS-PAGE gel under reducing conditions.
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![Flow Cytometry - Anti-VEGF Receptor 2 antibody [#4 (20I6)] (ab39378)](https://www.abcam.com/ps/products/39/ab39378/Images/ab39378-316620-anti-vegf-receptor-2-antibody-4-20i6-flow-cytometry.jpg)
Flow Cytometry - Anti-VEGF Receptor 2 antibody [#4 (20I6)] (ab39378)Flow cytometric analysis of HDLEC (primary human dermal lymphatic endothelial cells) labeling VEGF Receptor 2 using ab39378 at 5 μg/ml (red) compared to a negative control (unshaded).
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![Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [#4 (20I6)] (ab39378)](https://www.abcam.com/ps/products/39/ab39378/Images/ab39378-316619-anti-vegf-receptor-2-antibody-4-20i6-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [#4 (20I6)] (ab39378)HUVEC (human umbilical vein endothelial cell line) cells stained for VEGF Receptor 2 (green) using ab39378 at 7.5 μg/ml in ICC/IF. The nuclear counter stain is DAPI (blue).
A Goat anti-Mouse Alexa Fluor®488 conjugate was used as secondary antibody at 1/600 dilution.
