Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679)
![Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679)](https://www.abcam.com/ps/products/221/ab221679/Images/ab221679-307751-anti-vegf-receptor-2-antibody-epr21884-290-western-blot.jpg "Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21884-290] to VEGF Receptor 2
- Suitable for: WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-VEGF Receptor 2 antibody [EPR21884-290]
See all VEGF Receptor 2 primary antibodies -
Description
Rabbit monoclonal [EPR21884-290] to VEGF Receptor 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat placenta and lung lysates; bEnd.3 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21884-290 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679)All lanes : Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679) at 1/1000 dilution
Lane 1 : Mouse placenta lysate at 20 µg
Lane 2 : Rat placenta lysate at 20 µg
Lane 3 : Rat lung lysate at 20 µg
Lane 4 : Mouse lung lysate at 20 µg
Lane 5 : bEnd.3 (mouse brain endothelioma cell line) whole cell lysate at 10 µg
Secondary
Lanes 1-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 152 kDa
Observed band size: 180-230 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1-2: 3 minutes; Lane 3: 37 seconds; Lane 4: 59 seconds; Lane 5: 15 seconds
The molecular mass observed is consistent with the literature (PMID: 23354168 ; PMID: 20606037).
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![Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679)](https://www.abcam.com/ps/products/221/ab221679/Images/ab221679-2-benefits-of-recombinant-antibodies.png)
Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679)
