Anti-VEGF Receptor 2 antibody (ab11939)
Key features and details
- Rabbit polyclonal to VEGF Receptor 2
- Suitable for: Sandwich ELISA, WB, Flow Cyt, IHC-Fr
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-VEGF Receptor 2 antibody
See all VEGF Receptor 2 primary antibodies -
Description
Rabbit polyclonal to VEGF Receptor 2 -
Host species
Rabbit -
Specificity
The polyclonal antibody will detect native and recombinant human VEGFR2/KDR in ELISA experiments and on the surface or solubilized from different human cell types. -
Tested applications
Suitable for: Sandwich ELISA, WB, Flow Cyt, IHC-Frmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human VEGF Receptor 2. Highly pure recombinant human soluble extracellular domain of KDR (110 kDa) (D1-D7).
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Positive control
- IHC: Human spleen Flow: HUVEC and HDLEC cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
This antibody is affinity purified from rabbit sera. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.
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All lanes : Anti-VEGF Receptor 2 antibody (ab11939)
Lane 1 : recombinant human soluble VEGFR-2
Lane 2 : recombinant native VEGFR-2
Secondary
All lanes : anti-rabbit AP-conjugated antibody
Predicted band size: 151 kDaSamples were loaded in 10% SDS-PAGE under reducing conditions.
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Flow Cytometry analysis of primary human dermal lymphatic endothelial cells (HDLEC) labelling VEGF Receptor 2 with ab11939.
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Flow Cytometry analysis of primary human umbilical vein endothelial cells (HUVEC) labelling VEGF Receptor 2 with ab11939.
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Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.
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Standard curve for VEGF Receptor 2 (Analyte: ab54347); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGF Receptor 2 (ab11939) at 0.5µg/ml.