Human Fatty Acid Synthase ELISA Kit (ab279412)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.3 ng/ml
- Range: 5.5 ng/ml - 350 ng/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Fatty Acid Synthase ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 4.3% Inter-assay Sample n Mean SD CV% Extract 4 5.7% -
Sample type
Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
3.3 ng/ml -
Range
5.5 ng/ml - 350 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell Lysate 111 102% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Fatty Acid Synthase ELISA kit (ab279412) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Fatty Acid Synthase protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fatty Acid Synthase with 3.3 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Fatty acid synthase, also known as Type I fatty acid synthase, is an multienzyme encoded by the FASN gene. FASN contains two identical subunits in a head-to-tail connection of a 272 kDa monomer, each containing seven known catalytic activities, including ketoacyl synthase, malonyl/acetyltransferase, dehydrase, enoyl reductase, ketoacyl reductase, acyl carrier protein, and thioesterase. Fatty acid synthase catalyzes the biosynthesis of long-chain saturated fatty acids, particularly C16 palmitate, from acetyl-CoA and malonyl-CoA in the presence of NADPH.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Fatty Acid Synthase Capture Antibody 1 x 600µl 10X Human Fatty Acid Synthase Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human Fatty Acid Synthase Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Function
Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. -
Tissue specificity
Ubiquitous. Prominent expression in brain, lung, and liver. -
Sequence similarities
Contains 1 acyl carrier domain. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
Alternative names
- [Acyl-carrier-protein] S acetyltransferase
- [Acyl-carrier-protein] S malonyltransferase
- 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase
see all -
Database links
- Entrez Gene: 2194 Human
- Omim: 600212 Human
- SwissProt: P49327 Human
- Unigene: 83190 Human
Images
-
SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Recombinant Antibody benefitsTo learn more about the advantages of recombinant antibodies see here.
-
Example of human Fatty Acid Synthase standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.The Fatty Acid Synthase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native Fatty Acid Synthase in human HEK293T cell extract and based on a 25 µg/mL extract load.The concentrations of Fatty Acid Synthase were measured in duplicate and interpolated from the Fatty Acid Synthase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).