Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-COX7B antibody [EPR9326(B)] (ab140629)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9326(B)] to COX7B
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-COX7B antibody [EPR9326(B)]
    See all COX7B primary antibodies

  • Description

    Rabbit monoclonal [EPR9326(B)] to COX7B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human COX7B aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, 293T and MCF7 cell lysates; fetal kidney and fetal heart tissue lysates. IHC-P: Human bladder carcinoma and Human kidney tissue. ICC/IF: 293 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-COX7B antibody [EPR9326(B)] (ab140629)
    Western blot - Anti-COX7B antibody [EPR9326(B)] (ab140629)
    All lanes : Anti-COX7B antibody [EPR9326(B)] (ab140629) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : COX7B knockout HeLa cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 9 kDa
    Observed band size: 13 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab140629 observed at 13 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab140629 Anti-COX7B antibody [EPR9326(B)] was shown to specifically react with COX7B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265549 (knockout cell lysate ab257897) was used. Wild-type and COX7B knockout samples were subjected to SDS-PAGE. ab140629 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-COX7B antibody [EPR9326(B)] (ab140629)
    Western blot - Anti-COX7B antibody [EPR9326(B)] (ab140629)
    All lanes : Anti-COX7B antibody [EPR9326(B)] (ab140629) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : Fetal kidney tissue lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : Fetal heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 9 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9326(B)] (ab140629)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9326(B)] (ab140629)

    Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labelling COX7B with ab140629 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9326(B)] (ab140629)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9326(B)] (ab140629)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling COX7B with ab140629 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-COX7B antibody [EPR9326(B)] (ab140629)
    Immunocytochemistry/ Immunofluorescence - Anti-COX7B antibody [EPR9326(B)] (ab140629)
    Immunofluorescent staining of 293 cells labelling COX7B with ab140629 at 1/250 dilution.
  • Anti-COX7B antibody [EPR9326(B)] (ab140629)
    Anti-COX7B antibody [EPR9326(B)] (ab140629)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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