Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-COX7B antibody [EPR9326(B)] (ab140629)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9326(B)] to COX7B
Suitable for: WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-COX7B antibody [EPR9326(B)]
See all COX7B primary antibodies
Description

Rabbit monoclonal [EPR9326(B)] to COX7B
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human COX7B aa 1-100. The exact sequence is proprietary.
Positive control
- WB: HeLa, 293T and MCF7 cell lysates; fetal kidney and fetal heart tissue lysates. IHC-P: Human bladder carcinoma and Human kidney tissue. ICC/IF: 293 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9326(B)
Isotype

IgG
Research areas

Western blot - Anti-COX7B antibody [EPR9326(B)] (ab140629)

All lanes : Anti-COX7B antibody [EPR9326(B)] (ab140629) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : COX7B knockout HeLa cell lysate
Lane 3 : MCF7 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 9 kDa
Observed band size: 13 kDa
why is the actual band size different from the predicted?

Lanes 1-3: Merged signal (red and green). Green - ab140629 observed at 13 kDa. Red - loading control ab8245 observed at 36 kDa.

ab140629 Anti-COX7B antibody [EPR9326(B)] was shown to specifically react with COX7B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265549 (knockout cell lysate ab257897) was used. Wild-type and COX7B knockout samples were subjected to SDS-PAGE. ab140629 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-COX7B antibody [EPR9326(B)] (ab140629)

All lanes : Anti-COX7B antibody [EPR9326(B)] (ab140629) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : Fetal kidney tissue lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Fetal heart tissue lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 9 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9326(B)] (ab140629)

Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labelling COX7B with ab140629 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX7B antibody [EPR9326(B)] (ab140629)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling COX7B with ab140629 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-COX7B antibody [EPR9326(B)] (ab140629)

Immunofluorescent staining of 293 cells labelling COX7B with ab140629 at 1/250 dilution.
Anti-COX7B antibody [EPR9326(B)] (ab140629)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com