Anti-ENSA antibody [EPR8008(2)] (ab180513)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8008(2)] to ENSA
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ENSA antibody [EPR8008(2)]
See all ENSA primary antibodies -
Description
Rabbit monoclonal [EPR8008(2)] to ENSA -
Host species
Rabbit -
Specificity
ENSA (O43768) and ARPP-19 (P56211) -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB MouseRatHuman -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ENSA aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: O43768-2 -
Positive control
- K562 cells, HeLa whole cell lysate (ab150035), Fetal brain, Rat and Mouse heart tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8008(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ENSA antibody [EPR8008(2)] (ab180513) at 1/10000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Mouse heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution
Predicted band size: 13 kDaBlocking/dilutin buffer 5% NFDM/TBST
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Anti-ENSA antibody [EPR8008(2)] (ab180513) at 1/2000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated Secondary antibody at 1/1000 dilution
Predicted band size: 13 kDaBlocking/diuting buffer buffer 5% NFDM/TBST
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Flow cytometric analysis of K562 cells labeling ENSA with ab180513 at 1/70 dilution. Goat anti rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Cells were fixed in 2% paraformaldehyde.
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