Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 10, 2021

Anti-ENSA antibody [EPR8008(2)] (ab180513)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8008(2)] to ENSA
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-ENSA antibody [EPR8008(2)]
See all ENSA primary antibodies
Description

Rabbit monoclonal [EPR8008(2)] to ENSA
Host species

Rabbit
Specificity

ENSA (O43768) and ARPP-19 (P56211)

Tested Applications & Species

Application	Species
Flow Cyt	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ENSA aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: O43768-2
Positive control
- K562 cells, HeLa whole cell lysate (ab150035), Fetal brain, Rat and Mouse heart tissue lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR8008(2)
Isotype

IgG
Research areas

Western blot - Anti-ENSA antibody [EPR8008(2)] (ab180513)

All lanes : Anti-ENSA antibody [EPR8008(2)] (ab180513) at 1/10000 dilution

Lane 1 : Fetal brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Mouse heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution

Predicted band size: 13 kDa

Blocking/dilutin buffer 5% NFDM/TBST
Western blot - Anti-ENSA antibody [EPR8008(2)] (ab180513)

Anti-ENSA antibody [EPR8008(2)] (ab180513) at 1/2000 dilution + HeLa cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated Secondary antibody at 1/1000 dilution

Predicted band size: 13 kDa

Blocking/diuting buffer buffer 5% NFDM/TBST
Flow Cytometry - Anti-ENSA antibody [EPR8008(2)] (ab180513)

Flow cytometric analysis of K562 cells labeling ENSA with ab180513 at 1/70 dilution. Goat anti rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Cells were fixed in 2% paraformaldehyde.
Anti-ENSA antibody [EPR8008(2)] (ab180513)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com